Lipofectamine? 2000 轉染試劑

Lipofectamine? 2000轉染試劑是一種多用途轉染試劑，可在各種貼壁和懸浮細胞系中提供高效轉染。研究人員可使用Lipofectamine? 2000試劑進行基于siRNA和shRNA的基因敲除實驗及基因表達研究。Lipofectamine? 2000是Life Technologies提供的用于siRNA和質粒DNA共轉染的較佳試劑。

采用Lipofectamine? 2000試劑，您可以實現：

? 在各種細胞系中具有好的轉染效率和高重組蛋白表達水平 (表1)

? 出眾的siRNA和質粒DNA共轉染性能

? 在血清存在時亦具有可靠的轉染效率 – 轉染后無需更換培養(yǎng)基

? 適用于高通量應用的可靠性能

? 建立穩(wěn)定細胞系的優(yōu)選

適用于基因表達和基因沉默的高性能轉染試劑

Lipofectamine? 2000試劑適合所有常見細胞系及許多難以轉染的細胞系，可用于含有或不含血清的培養(yǎng)基。在基因沉默應用中，Lipofectamine? 2000試劑的高效轉染提供了很高的基因敲除水平，足以獲得令人信服的結果。Lipofectamine? 2000試劑可實現siRNA和質粒DNA的高效轉染，它是共轉染的優(yōu)選試劑。Lipofectamine? 2000試劑使用方便 – 只需將其與核酸混合，加入細胞培養(yǎng)物即可。

適合高通量操作

Lipofectamine? 2000試劑使用簡單、快速且具有很高的轉染效率，適用于瞬時蛋白表達或高通量RNAi轉染。在上述應用中，可輕松建立自動化或機器人系統(tǒng)轉染條件。如需了解有關Lipofectamine? 2000的實驗方案、文獻引證及其它產品信息，可登錄細胞系數據庫。

Lipofectamine?2000轉染方案/質粒轉染

脂質體胺? 2000轉染示例

引用和文獻：

Molecular and Biochemical Characterization of Rat epsilon -N-Trimethyllysine Hydroxylase, the First Enzyme of Carnitine Biosynthesis.

• 作者:Vaz F M; Ofman R; Westinga K; Back J W; Wanders R J;

• 期刊:J Biol Chem

• PubMed ID:11431483

A novel ankyrin repeat-containing gene (Kank) located at 9p24 is a growth suppressor of renal cell carcinoma.

• 作者:Sarkar Shubhashish; Roy Badal Chandra; Hatano Naoya; Aoyagi Teiichiro; Gohji Kazuo; Kiyama Ryoiti;

• 期刊:J Biol Chem

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Naturally occurring mutations in the largest extracellular loops of ABCA1 can disrupt its direct interaction with apolipoprotein A-I.

• 作者:Fitzgerald ML, Morris AL, Rhee JS, Andersson LP, Mendez AJ, Freeman MW.

• 期刊:J Biol Chem

• PubMed ID:12084722

The identification and characterization of a noncontinuous calmodulin-binding site in noninactivating voltage-dependent KCNQ potassium channels.

• 作者:Yus-Najera Eva; Santana-Castro Irene; Villarroel Alvaro;

• 期刊:J Biol Chem

• PubMed ID:12032157

cAMP-dependent protein kinase regulates desensitization of the capsaicin receptor (VR1) by direct phosphorylation.

• 作者:Bhave Gautam; Zhu Weiguo; Wang Haibin; Brasier D J; Oxford Gerry S; Gereau Robert W 4th;

• 期刊:Neuron

• PubMed ID:12194871

Human apical sodium-dependent bile salt transporter gene (SLC10A2) is regulated by the peroxisome proliferator-activated receptor alpha.

• 作者:Jung Diana; Fried Michael; Kullak-Ublick Gerd A;

• 期刊:J Biol Chem

• PubMed ID:12055195

The activated glucocorticoid receptor modulates presumptive autoregulation of ribosomal protein S6 protein kinase, p70 S6K.

• 作者:Shah O Jameel; Iniguez-Lluhi Jorge A; Romanelli Angela; Kimball Scot R; Jefferson Leonard S;

• 期刊:J Biol Chem

• PubMed ID:11705993

Transcriptional Regulation of Kaposi's Sarcoma-associated Herpesvirus-encoded Oncogene Viral Interferon Regulatory Factor by a Novel Transcriptional Silencer, Tis.

• 作者:Wang Xin-Ping; Zhang Yan-Jin; Deng Jian-Hong; Pan Hong-Yi; Zhou Fu-Chun; Gao Shou-Jiang;

• 期刊:J Biol Chem

• PubMed ID:11821384

A highly effective dominant negative alpha s construct containing mutations that affect distinct functions inhibits multiple Gs-coupled receptor signaling pathways.

• 作者:Berlot Catherine H;

• 期刊:J Biol Chem

• PubMed ID:11927592